Structural features of ghrelin-producing endocrine cells and their reactive changes after experimental stress

The work is devoted to the analysis of reactive changes in ghrelin-producing endocrine cells (HPEC) main areas production this regulatory peptide – stomach and hypothalamus during modeling psycho-emotional stress after replacement therapy. Reactive HPECs experimental post disorder models have not previously been specifically studied. Meanwhile, practice establish effectiveness therapeutic interventions suggests feasibility obtaining new informative criteria for assessing degree its impairment effect. purpose study was quantitative features GPEC a model psychogenic stress. Material methods. We studied paraffin sections mucous membrane cardial part four groups mature male rats. first group (n=5) consisted intact rest animals were subjected single emotional In nostrils two (n=5 each), 12 hours stress, 20 μg solution ghrelin (GHRL, Tocris, Gr. Brit.) antagonist receptors ([D-Lys3]-GHRP-6, Brit.). On 4th day, selected control (n=5). number endocrinocytes lateral nuclei arcuate complex counted over an area 0,01 mm2, fundic glands 0,015 mm2; endocrinocyte bodies determined using Imagescope program (Electronic analysis, Russia). median, upper lower quartiles, significance their differences compared (using nonparametric Mann–Whitney test) measured GraphPad PRISM 6.0 (GraphPad Software, USA). Differences considered significant at p<0,01. Results. During short-term experience viable gastric hypothalamic decreased by 2,5 3,8 times (respectively; p<0.001), body 1,5 1,6 (p<0,001). Pharmacological methods correction did significantly affect these parameters HPEC (p>0,05).